To further elucidate the molecular mechanism by which metformin inhibits the proliferation of cervical cancer cells, SiHa and HeLa cells were treated with varying concentrations of metformin (0 mM, 5 mM, 10 mM, and 20 mM) for 48 h, followed by western blotting to detect PI3K (p110), p-PI3K p85 (Tyr199), Akt, and p-Akt (ser473) protein expression. This evidence concerns the gene AKT1 and cervical cancer.