To determine whether NCSTN-induced phenotypes and nuclear translocation of β-catenin in HCC cells was also dependent on Notch/AKT signaling pathway, we first evaluated the expression of Notch intracellular domain (NICD) and phosphorylated AKT (p-AKT) in HCCLM3 and Hep3B cells. This evidence concerns the gene AKT1 and hepatocellular carcinoma.