We note that the H3K27ac peaks within THADA are much stronger than those upstream of IGF2BP3 in the absence of the translocation (Additional file 1: Fig. S2c), suggesting that the fusion brings a strong set of enhancers upstream of IGF2BP3. Selecting ten regions (1 to 1.5 kb) that show strong H3K27ac enrichment upstream of the majority of the breakpoints (Fig. 4e and Additional file 1: Fig. S6c), we performed dual-luciferase reporter assays in two thyroid cancer cell lines FTC238 and FTC133 (Fig. 4e, Fig. 4f and the “Methods” section). Here, IGF2BP3 is linked to thyroid gland carcinoma.