Thus, our finding that overexpression of NR2F2 promoted the EMT in MCF-7 cells, is consistent with other reports, which found that NR2F2 is highly expressed in an invasive colon cancer cell line HT29, compared with a normal colonic cell line CCD-18Co and that NR2F2 regulated the EMT through crosstalk with TGF-β signalling [43]. Here, TGFB1 is linked to malignant colon neoplasm.