We have further refined and validated this diet formula, termed the Amylin liver NASH (AMLN) diet [12], for reliably inducing metabolic, biochemical and liver-biopsy confirmed histological changes recapitulating hallmarks of NASH in C57BL/6 J (AMLN DIO-NASH) and leptin-deficient ob/ob (AMLN ob/ob-NASH) mice. Here, LEP is linked to metabolic dysfunction-associated steatohepatitis.