Twenty-five out of twenty-eight drug target-associated genes were significantly regulated in GAN DIO-NASH mice, including downregulation of ACACA/ACC1, ACACB/ACC2, DGAT1, HMGCR, KHK, MAP 3 K5/ASK1, MTOR, NR1H3/LXR-α, NR1H4/FXR, THRB and upregulation of CCR2, CCR5, CYSLTR1, FGF21, LGALS3/MAC-2, LOXL2, PPARG, SLC10A2/IBAT, SERPINH1/HSP47, TLR4, TNF/TNF-α. This evidence concerns the gene KHK and metabolic dysfunction-associated steatohepatitis.