A protective in vivo role of PrPC via Akt in ischemia was demonstrated by Weise et al., who showed that PrP0/0 mice subjected to transient (60 min) or permanent ischemia presented with a decrease of phosphorylated Akt (pAkt), leading to enhanced caspase-3 activation 6 h after reperfusion when compared to WT mice [51]. Here, AKT1 is linked to ischemia.