To further explore the role of SCD-1 upregulation in MG63 osteosarcoma cells under 20 dynes/cm2 shear force stimulation, cells were kept as the controls or stimulated with 20 dynes/cm2 shear force for 1, 4, 8 and 24 h, and their differentiation markers, i.e., Runx2 and osteocalcin (OCN), were analyzed. The gene discussed is SCD; the disease is osteosarcoma.