Our results about the inhibition of SCD-1 and its transcription regulator, i.e., PPARδ, in MG63 osteosarcoma cells could recover the high shear force-inhibited gene expression of differentiation markers and might provide a new hint if SCD-1 activity and expression regulation could also be applied in differentiation promotion therapy of osteosarcoma. Here, PPARD is linked to osteosarcoma.