CFTR function can be rescued by either editing the endogenous gene or inserting a wild type CFTR at a safe harbor locus such as AAVS1. The three most prominent gene editing techniques that have been explored as potential CF treatment approaches are zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated systems (Cas). Here, CFTR is linked to cystic fibrosis.