To further maintain the functionality of ICC in vitro, STO (SIM, Sandos Inbred Mouse) embryonic fibroblast feeder cells have been co-cultured with enriched murine Kit+ ICC (purified from primary mouse intestinal smooth muscle cell mixture through the use of magnetic-activated cell sorting and an anti-Kit antibody) [66]. Here, KIT is linked to intrahepatic cholangiocarcinoma.