Furthermore, Chang et al. demonstrated that metformin—which has previously been shown to reduce cyst growth and fluid secretion in 3D cell culture assays, murine embryonic kidney cultures, and rodent models of ADPKD—also reduces pronephric cyst formation and dorsal body curvature in zebrafish Pkd2 morphants [142]. The gene discussed is PKD2; the disease is autosomal dominant polycystic kidney disease.