Higher than expected frequencies of P/LP variants were found in genes previously linked to osteosarcoma, e.g., cyclin dependent kinase inhibitor 2A (CDKN2A), menin 1 (MEN1), Von Hippel-Lindau (VHL) tumor suppressor, protection of telomeres 1 (POT1), APC, mutS homolog 2 (MSH2), ATRX chromatin remodeler (ATRX) and TP53. Here, MSH2 is linked to osteosarcoma.