PPI network analysis demonstrated that two NB-specific immune-related genes, GAL and TWIST1, directly connected to TP53, while ADAM22 interacted with TP53 through TWIST1, which suggested that UHR-NB was a P53 related subtype, although the molecular mechanisms of TP53 in UHR-NB were still poorly understood. This evidence concerns the gene TWIST1 and neuroblastoma.