qRT-PCR validation at the different time points revealed that all candidate genes, except KLF10, which was up-regulated at early but down-regulated at later time point, were not significantly up- or down-regulated at the early time point (24 h), suggesting that the biologic effects of these genes at later time point could be linked to the resistance and persistence of AML cells after combination therapy (Figure 5E). The gene discussed is KLF10; the disease is acute myeloid leukemia.