CDC42 and neoplasm: We saw a range of expected phenotypes, for example, increased clonal coverage following RNAi of the known tumor suppressor, Tsc1 (a negative regulator of Tor signaling); reduced clonal coverage following RNAi of a known promoter of the cell cycle, tkv (promotes Dpp signaling); increased multilayering following RNAi of the polarity determinants scrib, expanded, and dlg; and smaller apices following RNAi of Cdc42, as has been observed previously (Georgiou et al., 2008) (Table S1).