However, another study showed that pre‐treatment with alpha‐mangostin partly inhibited the activation of autophagic cell death via down‐regulation of p‐mTOR, p‐AKT and the LC3II/LC3I ratio, subsequently decreasing the release of inflammatory factors, including tumour necrosis factor (TNF)‐α and IL‐1β, and inhibiting the activation of apoptotic pathways in APAP‐induced ALI models.39 The gene discussed is TNF; the disease is acute respiratory distress syndrome.