The results of a previous in vivo study were consistent with the data from the present study, observing the overexpression of SIRT1 in response to hyperglycemia; the overexpression of SIRT1 significantly decreased the expression levels of senescence-associated markers, such as p53, p21 and plasminogen activator inhibitor-1 (38). This evidence concerns the gene TP53 and Hyperglycemia.