In fact, our results were supported by those of Angelo et al. [20] and Mahmoud et al. [48] who have also observed high percentages of CD4+CD25+ and CD4+Th17 cells in insulin-treated women with GDM, suggesting that insulin treatment did not influence the proportion of these cells in pregnant women with in type 2 diabetes as well as in women with GDM [49]. Here, INS is linked to type 2 diabetes mellitus.