The serum levels of liver enzymes (ALT, AST, ALP, and GGT), bilirubin (TBIL and DBIL), total bile acids (TBA), globulin (GLB), IgM, and IgG were significantly elevated in patients with PBC-AIH OS (both noncirrhotic and cirrhotic) when compared to healthy control individuals. Here, CD40LG is linked to primary biliary cholangitis.