Moreover, low PRKACB levels significantly contributed to worse OS in CRC patients without non-mucinous cancers (HR=2.557(1.328-4.924), log-rank P=0.0108, Figure 4E) and the down-regulation of PRKACB was observed in patients with stage III-IV colorectal cancer (HR = 2.931 (1.357-6.333), log-rank P = 0.0145, Figure 4G), but not identified as risk factors for patients with stage I-II colorectal cancer (P> 0.05, Figure 4H). The gene discussed is PRKACB; the disease is colorectal cancer.