Results showed that both HMGB1-RAGE (p < 0.001, n = 3) and HMGB1-TLR4 (p < 0.01, n = 3) interactions were significantly high in the cerebral cortex and hippocampus of NASH mouse brain (Fig. 5b). This evidence concerns the gene TLR4 and metabolic dysfunction-associated steatohepatitis.