MCD fed mouse group representing NASH showed at least a 2-fold increased co-localization events of p47phox and gp91 phox when compared to lean control (CHOW diet) (p = 0.007) (Fig. 6e, f) suggesting a strong NADPH oxidase 2 (NOX-2) activation. This evidence concerns the gene CYBB and metabolic dysfunction-associated steatohepatitis.