BDNF and metabolic dysfunction-associated steatohepatitis: Our results showed that there was significantly decreased BDNF gene expression (to nearly 50%, p < 0.01, n = 3) (Fig. 3c) and protein expression, as indicated by immunoreactivity in immunohistochemistry (to nearly 2.5%, p < 0.001, n = 3) (Fig. 3a, b) and immunoblot analysis (to nearly 50%, p < 0.001, n = 3) ( Fig. 3f, g) in brain tissue of MCD diet mouse group (NASH) compared to CHOW fed mice (lean control).