Furthermore, using immunofluorescence microscopy, we found that there was a significant increase co-localization of Lcn2 with both CD11b and CD31 especially at cerebral cortex and hippocampus regions respectively compared to GFAP positive cells in MCD diet mouse group (Fig. 1e) which confirms glial and endothelial location of Lcn2 in NASH pathology. The gene discussed is PECAM1; the disease is metabolic dysfunction-associated steatohepatitis.