Remarkably, more than 15-fold increased immunoreactivity of phosphorylated Tau protein (phosphorylated at serine 396) was observed in brain tissue of MCD diet mouse group compared to CHOW diet fed (Fig. 3a, b) mouse group suggesting a clear implication of neurodegeneration and signs of AD in NASH murine model in the presence of increased circulatory Lcn2. This evidence concerns the gene LCN2 and metabolic dysfunction-associated steatohepatitis.