RB1 and glioblastoma: These samples displayed broad coverage of major driver mutations5, including EGFR, EGFRvIII (deletion in exon 2–7), TP53, RB1, PTEN, and PIK3CA (Fig. 1a), and copy number alterations (CNAs) in CDKN2A/TP53 (deletion) and EGFR/PDGFRA (amplification) (Supplementary Fig. 1a), indicating that these samples represented the GBM mutational spectrum.