To further validate the intratumoral heterogeneity of GPC subtypes at a single cell level, we used a tumor microarray (SMC-TMA) of independent IDH wild-type GBM tissues to measure relative expression of PHGDH (good prognostic marker representing GPC2, Fig. 4f) and Nestin (representing GPC1, Fig. 4a) by multiplex fluorescent immunohistochemistry that generated reliable signal intensities at a single cell resolution. This evidence concerns the gene NES and neoplasm.