We therefore aimed to apply our GBM proteomic subtype classification to single-cell transcriptome data reported by Darmanis et al.31, which comprised 3589 single cells from four patient IDH wild-type GBM tumors and provided information regarding the brain cell type (vascular, immune, neuronal, and glial) and three-dimensional location (tumor core, periphery) (see “Methods” and Fig. 5a). Here, IDH1 is linked to glioblastoma.