NLK immunopurified from mononuclear cells from the bone marrow aspirates of three DBA patients carrying different RPS19 mutations significantly increased phosphorylation of all three substrates compared with healthy control cells (2.2-, 2.0-, and 2.2-fold NLK phosphorylation (i), 3.3-, 2.4-, and 2.6-fold c-Myb phosphorylation (ii), and 4.8-, 4.1-, and 4.2-fold Raptor phosphorylation (iii)). Here, RPTOR is linked to Diamond-Blackfan anemia.