NG2 positive astrocytes have been reported in human AD in individuals with high Aβ plaque load (Nielsen et al., 2013), and comparison with NG2+/GFAP− OPCs and NG2−/GFAP+ astrocytes in the same field of view in our study indicates NG2+/GFAP+ cells had a morphology more akin to astrocytes than OPCs (Fig. 6D). Here, GFAP is linked to Alzheimer disease.