NFATC3 and familial dilated cardiomyopathy: We observed the nuclear translocation of the transcription factor NFATc3 by coimmunolocalization with DAPI (Supplemental Fig. S3) in cells transduced with DCM-causing mutations, whereas there was no evidence of NFATc3 in the nucleus compared with cytoplasmic background in WT-transduced cells (Fig. 6A).