A comparison of qPCR output expression of the common reference genes, 18S, Beta-Actin, GAPDH and B2M (Fig. 3A), oncogenes or drivers of cancer progression, CDKN1, p53 and p16 (Fig. 3B) and miRNA biogenesis genes, Dicer and Ago2 (Fig. 3C) was then performed across these 3 RT priming strategies. The gene discussed is GAPDH; the disease is cancer.