In this study, we found that GFAP gene and protein levels were significantly increased in the ACC of rats with IBS visceral hypersensitivity and electroacupuncture significantly inhibited GFAP gene and protein expression, similar to FCA therapy, which suggests that electroacupuncture significantly improved IBS visceral hypersensitivity by inhibiting astrocyte activation in ACC. This evidence concerns the gene GFAP and irritable bowel syndrome.