NPY and coinfection: Co-infection with NPY-pHluorin and TeNT (4–5 days before imaging, identified by IRES-mCherry expression tag) disrupted DCV exocytosis (DCV fusion events control: 57 ± 19, TeNT: 0.14 ± 0.08; Fig. 1F–H) but did not alter the number or sub-cellular distribution of DCVs (Supplementary Fig. S1).