For post-infection treatment, cells were infected with DENV 2 before being treated with the 5 VDR agonists at 0, 1, 3, 6 and 12 h post-infection (with 0 h representing treatment with the compounds immediately after the end of the infection step), and at 24 h post-infection the level of infection and the virus titer in the supernatant were again determined by flow cytometry and standard plaque assay respectively. The gene discussed is VDR; the disease is infection.