Therefore, we used GAD67-pTDP-43 double labelling to explore the vulnerability of GABAergic interneurons of the primary motor cortex to the accumulation of pTDP-43 aggregates (ALS n = 7, control n = 3; supplementary material), with the reasoning that this would allow us to obtain a histological window into potential imbalances of inhibitory and excitatory neurotransmission in ALS motor cortex. Here, GAD1 is linked to amyotrophic lateral sclerosis.