By qmIF and targeted genomic expression analysis, we confirmed in human breast cancer specimens the biologic activity of MK-2206, with well-described changes in gene expression of known targets of the PI3K/Akt/mTOR pathway as well as marked indirect immune related effects of Akt inhibition on the TME including an increase in CTL density as well as greater expression of interferon related genes and lower expression of myeloid genes. Here, AKT1 is linked to breast carcinoma.