In addition, several studies have demonstrated that EOMEShighT-Betlow characterizes exhausted T cells and immunotherapy resistance (9, 49, 50), and similarly we observed a significantly higher proportion of CD8+ T cells positive for nuclear EOMEShigh and significantly lower nuclear T-bet expression in PD-1+CD8+ T cells in immunotherapy-resistant melanoma patients (as defined by RECIST 1.1, Supplementary Figure 5A) and in PD-1+CD8+ T cells from TNBC patients (Figure 6A). This evidence concerns the gene PDCD1 and melanoma.