CT26 cell line is a typical example of colon cancer expressing mutant KRAS that is associated with the degree of aggressiveness of colon cancer due to its importance in affecting growth, proliferation, and differentiation of cells through mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade [48]. This evidence concerns the gene MAPK1 and malignant colon neoplasm.