The signal detection results showed: (a) HPV2 did not yield any signal; (b) HPV4 was associated with amenorrhea, FSH increased, menstrual irregularities and premature menopause, indicating HPV4 with a sataistical risk of POI and related events; (c) irregular menstruation was detected as signal for HPV9 only. Here, BRD2 is linked to amenorrhea.