After the characterization of the EV-derived MMP/TIMP protein content of HKC-8, 786-O, RCC-FG2 and Caki-1 cell lines, we quantified the mRNA levels of MMP-1, TIMP-1 and TIMP-2 inside the cells and in the cell-derived EVs. This evidence concerns the gene TIMP2 and renal cell carcinoma.