In urine samples, the urinary N-actyl-β-d-glucosaminidase (uNAG)/Cr levels had the greatest ability to discriminate the leptospirosis group from HCs based on the AUC (0.849), followed by uCLU/Cr (0.731), utr-OPN/Cr (0.667), and uFL-OPN (0.619) (Figure 2B). The gene discussed is SPP1; the disease is leptospirosis.