The result of ROC analysis of plasma samples (Figure 2A) shows that pFL-Gal-9 levels had the greatest ability to discriminate leptospirosis patients from HCs based on the AUC (0.953), followed by sCyC (0.934), sCr (0.892), pFL-OPN (0.875), and ptr-OPN (0.632) (Figure 2A). The gene discussed is SPP1; the disease is leptospirosis.