GPT and Hepatic steatosis: Various HF diets have been used in different rat strains to assess the development and progression of NAFLD as characterized by hepatic steatosis, elevated triglyceride levels, changes in alanine transaminase (ALT), and a spectrum of histopathological changes in the liver (as reviewed by Kucera and Cervinokova, 2014; Van Herck et al., 2017) [5, 6].