First, comparative analysis of hub gene expression in lung adenocarcinoma and adjacent normal tissue was performed in our study, and our results showed that the expression levels of BUB1B, CDC45, BUB1, TTK, TOP2A, NDC80, MCM2, and DTL were significantly higher in lung adenocarcinoma tissues than in adjacent normal lung tissues (P < 0.01). The gene discussed is CDC45; the disease is lung adenocarcinoma.