Using the modified rapid fluorometric ADCC (RFADCC) assay targeting green fluorescent protein (GFP)-CEM-NKR-CCR5-SNAP cells (29) coated with Bal gp120, derived from a clade not present in the vaccination regimen, both antibodies from natural infection and from vaccination showed similar maximum lysis ranging from 52.54% for CH55 to 58.4% for N12-i3 (Fig. 1A). This evidence concerns the gene ITIH4 and infection.