Ahrendt et al. (1999) [15] used four PCR-based techniques (p53 sequencing, K-ras mutation ligation assays, K-ras-enriched PCR, p16 methylation-specific PCR, and microsatellite analysis) to examine the presence of cancer cells in bronchoalveolar lavage (BAL) fluid. The gene discussed is TP53; the disease is cancer.