Additionally, it was reported that the levels of IL-6, TNFα, and IL-1β are significantly elevated in the GCF and gingival tissue of periodontitis patients, and the elevated serum levels of IL-6 TNFα and IL-1β decreased after nonsurgical periodontal therapy, resulting in a significant clinical improvement of the periodontal status [29,30]. This evidence concerns the gene TNF and periodontitis.