Regarding the overproduction of ROS in the hematopoietic stem-cell compartment of JAK2V617F knock-in mice, the application of the anti-oxidant N-acetylcysteine (NAC) could restore the normal phenotype in these mice, normalize peripheral blood parameters, decrease splenomegaly, reduce the number of JAK2-mutant hematopoietic stem and progenitor cells in the spleen and bone marrow and reduce DNA double-strand breaks being increased in JAK2V617F mutant MPN. Here, JAK2 is linked to Splenomegaly.