Data quantifying Tregs in breast cancer-TDLNs, is scarce18, and has been mainly obtained by FOXP3 detection by IHC that do not allow the distinction between the suppressive CD4+ CD25+ FOXP3high T and the non-suppressive CD4+ CD25+ FOXP3low- T cells12,18,19,50. This evidence concerns the gene FOXP3 and breast carcinoma.