CD4 and neoplasm: The cumulative frequency of TCR-β CDR3s in each sample (Fig. 5a, c and Supplementary Fig. 5a and 5c–d), indicates that Tregs were more clonally expanded than Tconvs in all tissues; and that the TCR repertoire of Tregs and Tconvs were less diverse in the tumor than in the TDLNs, suggesting an accumulation of tumor-specific CD4+ T cells in the tumor.