In our study, we verified that miR-526b-3p exhibited post-transcriptional regulatory effects on MMP2 and VEGFA expression by binding to its 3’UTR via dual-luciferase reporter assay, and in the meantime, miR-526b-3p suppressed glioma angiogenesis by specifically targenting and suppressing MMP2 and VEGFA. The gene discussed is VEGFA; the disease is central nervous system cancer.