The aim of the present study was to analyze the role of NG2/CSPG4 in relation to malignant progression and prognosis in a series of 61 adult human gliomas of different molecular subtypes and in nine GB-derived cell lines At the protein level, NG2/CSPG4 expression was evaluated by immunohistochemistry (IHC) in tumor tissues and by immunofluorescence (IF) in cell lines. Here, CSPG4 is linked to central nervous system cancer.