A group of screening data on gene expression profiles of HCT‐116 cells before and after piRNA‐823 intervention showed that molecule glucose‐6‐phosphate dehydrogenase (G6PD) gene related to glucose metabolism pathway in colorectal cancer cells changed significantly before and after piRNA‐823 intervention, and positively correlated with the content of piRNA‐823. The gene discussed is G6PD; the disease is colorectal cancer.