Twelve CF patients from Guayaquil (coast) and Cuenca (highland) who were available and had new CFTR disease-causing variants reported in a previous study were selected: one patient from Guayaquil with c.1473T>A:p.Cys491∗, one patient from Guayaquil and two from Cuenca with c.2672del:p.Asp891Alafs∗15, one patient from Cuenca with c.1486T>C:p.Trp496Arg, and six patients from Guayaquil and one from Cuenca with [c.757G>A:p.Gly253Arg; c.1352G>T:p.Gly451Val] were selected [18]. The gene discussed is CFTR; the disease is cystic fibrosis.