On the other hand, chromatin immunoprecipitation assays showed that E2 binds a region comprised between nucleotides 565-363 upstream the SRSF1 AUG starting site through its N-terminal domain and transactivates the SRSF1 expression in the U2OS human osteosarcoma cell line (Mole et al., 2009b). This evidence concerns the gene SRSF1 and osteosarcoma.