As shown in Figures 2D,E, the highest apoptotic level was detected and the apoptotic proteins of cleaved caspase 3, cleaved caspase 7, and cleaved caspase 9 but not cleaved caspase 8 were significantly increased in CDK8 knockdown CRC cells after IR treatment, suggesting that downregulation of CDK8 results in radiosensitivity primarily through the intrinsic apoptotic pathway. The gene discussed is CASP3; the disease is colorectal carcinoma.