SIRT1 and hepatocellular carcinoma: In a recent study, Rui E. Castro et al.134 proposed that microRNA-34a overexpression is one of the reasons for liver steatosis without inflammation toward steatohepatitis or HCC; microRNA-34a overexpression will increase p53 acetylation and transcription, which leads to the diminishment of sirtuin 1 (SIRT1) in the NAFLD liver.