The results of pre-chemotherapy evaluation showed that only 2 (2.04%) of the 98 examined patients (one patient with ALL and another with lymphoma), had a positive IgM titer (>1 IU/ml) and 46 cases (46.9%) had positive IgG titers (>15 IU/ml), Out of these 46 patients, 20, 15 and 11 were AML, ALL and lymphoma, respectively. Here, CD40LG is linked to acute lymphoblastic leukemia.